Composite

Part:BBa_K782084:Design

Designed by: Fedja Pavlovec   Group: iGEM12_Slovenia   (2012-09-24)


10x[TALA] operator_CMV promoter_TALB:NLS:KRAB_t2a_mNeptune


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 720
    Illegal BamHI site found at 3773
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 147
    Illegal NgoMIV site found at 507
    Illegal AgeI site found at 12
    Illegal AgeI site found at 347
    Illegal AgeI site found at 372
    Illegal AgeI site found at 707
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4139
    Illegal BsaI.rc site found at 4688
    Illegal BsaI.rc site found at 4877
    Illegal SapI.rc site found at 4103
    Illegal SapI.rc site found at 4259


Design Notes

design


Source

source

References